Lipidomics: Methods and Protocols [Hardcover]

This fully updated book presents an account of areas of utility, techniques, and bioinformatic advancements in the field of lipidomics. Beginning with protocols for lipid isolation and extraction, the volume continues with techniques from extractive mass spectrometry to imaging mass spectrometry methods allowing localization of lipids in tissues. These protocols have been complemented by methods addressing specific problems from membranes, fractionated subcellular compartments or organelles to whole organisms. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls. Isolation of Mitochondrial Lipids and Mass Spectrometric Analysis.- Isolation of Neuronal Synaptic Membranes by Sucrose Gradient Centrifugation.- Multi-Macromolecular Extraction from Endosymbiotic Anthozoans.- High Resolution Liquid Chromatography-Mass Spectrometry for Lipidomics.- Characterization of Gangliosides from Mouse Optic Nerve Samples Using Mass Spectrometry.- Protocol and Methods Applicable to Retinal Vascular Diseases.- Analysis and Annotation of Phospholipids by Mass Spectrometry-Based Metabolomics.- Profiling the Mammalian Lipidome by Quantitative Shotgun Lipidomics.- Lipidomics Analysis with Triple Quadrupole Mass Spectrometry.- High-Performance Chromatographic Separation of Cerebrosides.- Quantification of Endocannabinoids from Biological Samples Using Liquid ChromatograplS%