PCR Primer Design [Hardcover]

This third edition provides new and updated chapters on design PCR primers for successful DNA amplification. Chapters are divided into seven parts, including primer design strategies for quantitative PCR, genotyping, multiplex PCR, in silico PCR primer design, and primer design to identify plant and animal viruses. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, PCR Primer Design, Third Edition aims to be useful for various fields of molecular biology, including biotechnology, molecular genetics, and recombinant DNA technology.

The significance of PCR primer design in genetic diversity studies; exemplified by recent research into the genetic structure of marine species.- Enhancing cohort PASA efficiency from lessons assimilated by mutant genotyping in C. elegans.- Design of oligonucleotides for allele-specific amplification based on PCR and isothermal techniques.- Detection of rubella virus by tri-primer RT-PCR assay and genotyping by fragment RT-PCR.- Design of mismatch primers to identify and differentiate closely related (sub)species  application to the authentication of meat products.- Primer design for the analysis of closely related species  application of non-coding mtDNA and cpDNA sequences.- Designing PCR primers for the amplification-refractory mutation system.- Validation of circular RNAs by PCR.- Primer Designing for Amplifying an AT-Rich Promoter from Al£“