Peptide Conjugation: Methods and Protocols [Paperback]

This volume explores diverse protocols for peptide conjugation, and provides thoroughly tested and scientifically valid techniques that allow researchers and scientists to prepare, purify, characterize, and use peptide conjugation methods for chemical, biochemical, and biological studies. Some of the topics discussed in this book are gold nanoparticles, proteins, pegylated lipids, and vitamins. Chapters also cover enzymatic ligation using sortase A, construction of a phage-displayed cyclic-peptide library, quantum dot-peptide conjugates, and preparation of lipopeptides by CLipPA chemistry. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Cutting-edge and comprehensive, Peptide Conjugation: Methodsand Protocols is a valuable resource for experienced researches and undergraduate students alike who are interested in learning more about this exciting and developing field. 

Peptide-Polymer Conjugation via Copper-Catalyzed Alkyne-Azide 1,3-Dipolar Cycloaddition.- Conjugation of Peptides to Gold Nanoparticles.- Peptide-Protein Conjugation and Characterization to Develop Vaccines for Group A Streptococcus.- Oxime/Hydrazone Conjugation at Histidine: Late-Stage Functionalization Approach of Unprotected Peptides.- Modification of Nanoparticles with Transferrin for Targeting Brain Tissues.- Peptide-Pegylated Lipid Conjugation Via Copper-Catalyzed Alkyne-Azide 1,3-Dipolar Cycloaddition.- Vitamin B12 - Peptide Nucleic Acid Conjugates.- Enzymatic Ligation of Disulfide-Rich Animal Venom Peptidl3¨