Plant Mitochondria: Methods and Protocols [Hardcover]

This detailed volume presents a wide range of techniques for plant mitochondrial analysis, ranging from tried-and-tested work horse techniques to the latest innovations. Within its pages, it explores subjects such as affinity-based isolation of mitochondria with magnetic beads, mitochondrial quality assessment protocols, measurement of uptake and release of specific metabolites, mitochondrial protein identification and visualization, as well as gene splicing and editing, and much more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Purification of Leaf Mitochondria from Arabidopsis thaliana Using Percoll Density Gradients.- Cell Type-Specific Isolation of Mitochondria in Arabidopsis.- Isolation of Plant Mitochondria Using Affinity Purification.- Isolation of Highly Purified, Intact, and Functional Mitochondria from Potato Tubers Using a Two-in-One Percoll Density Gradient.- Integrity Assessment of Isolated Plant Mitochondria.- High-Throughput Oxygen Consumption Measurements in Leaf Tissue Using Oxygen Sensitive Fluorophores.- Assessment of Respiratory Enzymes in Intact Cells by Permeabilization with Alamethicin.- Assessing the Kinetics of Metabolite Uptake and Utilization by Isolated Mitochondria Using Selective Reaction Monitoring-Mass Spectrometry (SRM-MS).- Complexome Profiling of Plant Mitochondrial Fractions.- High Throughput BN-PAGE for MitochondrilÓC