This second edition provides a comprehensive laboratory manual on skeletal development and skeletal repair research utilizing mouse models. Chapters provide methods and protocols on the most current and cutting-edge techniques in skeletal development and repair, histological, cellular, and molecular techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge,
Skeletal Development and Repair: Methods and Protocols-Second Edition aims to ensure successful results in the further study of this vital field.
Overview of Skeletal Development.- Overview of Skeletal Repair (Fracture Healing and Its Assessment).- Advantages and Limitations of Cre Mouse Lines Used in Skeletal Research.- Generation of Closed Transverse Fractures in Small Animals.- A Mouse Femoral Ostectomy Model to Assess Bone Graft Substitutes.- Surgical Induction of Posttraumatic Osteoarthritis in the Mouse.- Parabiosis: Assessing the Effects of Circulating Cells and Factors on the Skeleton.- Murine Limb Bud Organ Cultures for Studying Musculoskeletal Development.- Murine Limb Explant Cultures to Assess Cartilage Development.- Renal Capsule Transplantation to Assay Angiogenesis in Skeletal Development and Repair.- MicroCT for Scanning and Analysis of Mouse Bones.- Four-Point Bending Testing for Mechanical Assessment of Mouse Bone Structural Properties.- Phenotyping Intact Mouse Bones using Bone CLARITY.- Processing and Sectioning Undecalcified Murine Bone Specimens.- Preparation of Thin Frozen Sections from Nonfixed And Undecalcified Harl3,
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