The Plant Microbiome: Methods and Protocols [Paperback]

This volume provides methods, protocols, and reviews that are useful for new and experienced plant microbiome researchers. Chapters guide readers through the investigation of microbiomes associated with seeds, sampling microbiomes from plant compartments and tissues, culture-based methods, culture-independent metabarcoding methods, methods to obtain DNA and perform metabarcoding, protocols to block PCR amplification from the plant host, qPCR-based methods, editing of specific genes in Bacillus genomes, and Streptomycetes and plant microbial indicators. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, The Plant Microbiome: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Studying Seed Microbiomes.- Sampling Microbiomes Associated with Different Plant Compartments.- Sampling of Bacteria Associated with Plant Vascular Tissues.- Sampling of Plant Material to Study Endophytes in Small, Large and Woody Plants.- Preparation of Samples for Characterization of Arbuscular Mycorrhizal Fungi.- Culture-based Methods for Studying the Bacterial Root Microbiome of Wheat.- Fast Screening of Bacteria for Plant Growth Promoting Traits.- Protists in the Plant Microbiome - An Untapped Field Of Research.- Plant-associated Fungi: Methods for Taxonomy, Diversity, and Bioactive Secondary Metabolite Bioprospecting.- Extraction and Purification of DNA from Wood at Various Stages of Decay for Metabarcoding of Wood-Associated Fungi.- Peptide Nucleic Acid (PNA) Clamps to Reduce Co-Amplification of Plant DNA during PCls.