In-Situ PCR Techniques [Paperback]

This book describes comprehensive step-by-step protocols for the delineation of genetic amplification and histological detection techniques. Each procedure has been tested and validated for its sensitivity, precision, and reproducibility, and the authors give advice on the design of primers for PCR applications and on optimizing these protocols for use with plant, insect, and prokaryotic cells.

Dieses Buch fa?t auf Empfindlichkeit, Genauigkeit und Reproduzierbarkeit gepr?fte und validierte Versuchsanleitungen f?r die Lokalisierung von DNA und RNA mittels in-situ-PCR-Verfahren zusammen. Die Autoren geben zahlreiche Hinweise zum Design von Primern f?r die Polymerase-Kettenreaktion und zur Optimierung der Versuchsbedingungen.

Overview;
Review of the PCR Technique;
Preliminary Solution-Based Reactions;
Preparation of Glass Slides and Tissues;
In Situ PCR: DNA and RNA Targets;
Special Applications of In Situ Amplification;
Hybridization Reactions;
Validation and Controls;
Materials and Methods;
Select Biography;
Appendices;
Index;
About the Authors.

Omar Bagasra is with Claflin University in Orangeburg, South Carolina, USA.In situ gene amplification techniques offer tremendous potential as aids to clinical diagnosis through their ability to detect a single copy of a specific microbial, neoplastic, messenger, or mutated nucleic acid sequence in a cell smear, cell suspension, tissue section, or chromosome. In situ hybridization, applied in combination with the polymerase chain reaction (PCR), can be expected to fuel accelerated developments in the understanding of embryogenesis, organogenesis, and cell differentiation, as well as the pathogenesis of numerous disease processes. But the procedures are cumbersome and fraught with potential variables, and experimental results are difficult to reproduce.<ls)