Intracellular Pathogens: Methods and Protocols [Hardcover]

This detailed volume presents cutting-edge approaches to study infection, immunity, and intervention strategies against intracellular pathogens. The book explores areas such as how intracellular pathogens infect and survive inside the phagocytes, immunopathological features of infection, evasion of host-induced immune responses, cellular crosstalk in the presence of intracellular pathogens, host innate and adaptive immunity, and much more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Intracellular Pathogens: Infection, Immunity, and Intervention.- Methods for Genomic Epidemiology of Bacterial Pathogens: Example Salmonella.- Phagocytosis.- Large Scale Culture and Plasmid Preparation Procedure for Low-Yield Bacmids Containing Full-Length SARS-CoV-2 cDNAs.- Metatranscriptomic RNA-Seq Data Analysis of Virus-Infected Host Cells.- Metabolic Analysis of Intracellular Pathogenic Bacteria Using NMR.- Quantitative Proteomics of the Intracellular Bacterial Pathogen Salmonella enterica Serovar Typhimurium.- Median Tissue Culture Infectious Dose 50 (TCID₅₀) Assay to Determine Infectivity of Cytopathic Viruses.- Applications of Quantitative PCR (qPCR) in Studies of Virus-Host Interactions.- Establishing Air-Liquid Interface (ALI) Airway Culture Models for Infectious Disease Research.- Pig Acute Phase Proteins as Non-Antibody Systemic Biomarkers of IntlӉ