This volume details protocols for computer-assisted design and experimental characterization of RNA nanostructures. Chapters guide readers through RNA nanotechnology, design and characterization of RNA nanostructures, assessment of immunology of nanomaterials, biosensing and drug delivery, various biomedical applications, and delivery approaches for therapeutic RNA nanoparticles./Written in the format of the highly successful/Methods in Molecular Biology/series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.
/ Authoritative and cutting-edge,/RNA Nanostructures: Design, Characterization, and Applications/aims/to address essential topics and concerns in the growing field of RNA nanotechnology.
Molecular Dynamics Simulations of RNA Motifs to Guide the Architectural Parameters and Design Principles of RNA Nanostructures.- Computer assisted design and characterization or RNA nanostructures.- Combining Experimental Restraints and RNA 3D Structure Prediction in RNA Nanotechnology.- Structural Characterization of Nucleic Acid Nanoparticles using SAXS and SAXS-driven MD.- Metalated Nucleic Acid Nanostructures.- Bioconjugation of Functionalized Oligodeoxynucleotides with Fluorescence Reporters for Nanoparticle Assembly.- Light-assisted drying for the thermal stabilization of nucleic acid nanoparticles and other biologics.- Preparation of Nucleic Acid Aptamer Functionalized Silver/Gold Nanoparticle Conjugates.- Thermodynamic Characterization of Nucleic Acid Nanoparticles Hybridization by UV Melting.- Structural Characterization of DNA Templated Silver Nanoclusters by Energy Dispersive Spectroscopy.- Small Volume lCP
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