DNA Damage Detection: Methods and Protocols [Hardcover]

This volume explores techniques in DNA damage analysis, important not only for understanding cellular health after exposure to genotoxic agents but also for assessing cellular ability of genome maintenance. The book explores traditional DNA damage measurement methods, as well as modern fluorescence-based analysis and high throughput automatic analysis methods. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Practical and timely, DNA Damage Detection: Methods and Protocols serves as an ideal guide for readers who are unfamiliar with DNA damage as well as experts seeking protocols to adapt to their own research.

Modified Alkaline Comet Assay to Detect Oxidative DNA Damage.- Radiation-Induced DNA Strand Break Detection by Gel Electrophoresis.- Quantitative Measurement of 8-Hydroxy-2′-Deoxyguanosine in Serum/Plasma and Urine Using an Enzyme-Linked Immunosorbent Assay.- Radiation-Induced Gamma-H2AX Foci StaininglÓÙ