This fully updated book collects protocols that reflect current investigations into how stem cell populations change during organismal aging. These methodologies are well-established and described in easy-to-follow fashion so as to be valuable for not only experts but also novices in this ever-developing area of stem cell research. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, Stem Cells and Aging: Methods and Protocols, Third Edition serves as an ideal guide for researchers working toward furthering our understanding of the aging process.
Protocols to Assess the Aging of Human Mesenchymal Stem Cells.- Analyzing Muscle Stem Cell Function Ex Vivo.- Tubular Aggregates as a Marker of Aging in Skeletal Muscle.- Fusion of Human Synovium-Derived Mesenchymal Stem/Stromal Cells with Primary Human Chondrocytes Using the Modified Adherence Method (MAM).-