Binding and Kinetics for Molecular Biologists [Paperback]

This handbook offers a practical guide to the principles of quantitative analysis in biological experiments. The material is primarily aimed at working molecular biologists, but the scope and clarity of presentation make it equally suitable as an introduction for students. Topics covered range from the basicssuch as measuring the concentrations of macromoleculesthrough considerations of binding constants and the kinetics of molecular interactions. The book ends with a thorough consideration of data analysis.This handbook offers a practical guide to the principles of quantitative analysis in biological experiments. The material is primarily aimed at working molecular biologists, but the scope and clarity of presentation make it equally suitable as an introduction for students. Topics covered range from the basicssuch as measuring the concentrations of macromoleculesthrough considerations of binding constants and the kinetics of molecular interactions. The book ends with a thorough consideration of data analysis.
Contents

1. Performing Quantitative Experiments with Biomolecules
2. Affinity Constants
3. Cooperativity in Binding
4. Rate Constants for Binding and Dissociation
5. Kinetics of Regulation and Cooperativity in Binding Reactions
6. Constants for a Two-step Binding Reaction
7. Data Analysis
Appendix 1: Equation Derivations
Appendix 2: Resources and References
IndexThis textbook provides a good introduction to the theory of binding kinetics. It supplies technical insight to proper affinity assay development. The volume is ideally suited for readers who seek background information prior to designing ligand-protein interaction assays.
The Quarterly Review of Biology