Lipid Signaling Protocols [Hardcover]

In this second edition methods are described to measure the synthesis of lipids such as the phosphoinositides, ceramides and sphingomyelin, as well as techniques to molecularly characterize the various kinases and phosphatases that regulate the intracellular metabolism of these lipids. Lipid Signaling Protocols, Second Edition guides readers through detailed experimental protocols, which are complimented by review chapters that highlight the technical considerations, challenges and potential pitfalls associated with using these laboratory-based approaches. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Lipid Signaling Protocols, Second Edition aims to ensure successful results in the further study of this vital field.

?

Method for Assaying the Lipid Kinase Phosphatidylinositol-5-phosphate 4-kinase ? in Quantitative High-Throughput Screening (qHTS) Bioluminescent Format.- Assaying Ceramide Synthase Activity In Vitro and in Living Cells using Liquid Chromatography-Mass Spectrometry.- Fluorescent Assays for Ceramide Synthase Activity.- Identification of the Interactome of a Palmitoylated Membrane Protein, Phosphatidylinositol 4-kinase type II Alpha.- Measurement of Long Chain Fatty Acyl-CoA synthetase Activity.- Qualitative and Quantitative in vitro Analysis of Phosphatidylinositol Phosphatase Substrate Specificity.- Luciferase Reporter Assays to Assess Liver X Receptor Transcriptional Activity.- Metabolically Biotinylated Reporters for Electron Microscopic Imaging of Cytoplasmic Membrane Microdomains.- Fluorescence Recovery after Photobleaching Analysis of the Diffusional Mobility lCl