Live-Cell RNA Imaging: Methods and Protocols [Hardcover]

This detailed volume presents a comprehensive guide to live-cell RNA imaging techniques and methodologies that have been successfully used to study the dynamics or function of various RNAs in cells. The topics covered encompass techniques including the detection of aptamer-tagged mRNA molecules utilizing fluorogenic proteins, monitoring endogenous mRNA via a split-protein-based probe within neuronal cells, sensitive detection of low-abundance RNAs employing catalytic hairpin assembly circuits, monitoring RNA dynamics using the MS2 system within plant cells, detection of miRNA utilizing DNA-MOF probes, and identification of RNA G-quadruplex structures or nucleolar RNA through fluorescent probes, among other advanced techniques. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

 

Authoritative and practical, Live-Cell RNA Imaging: Methods and Protocols provides researchers with a diverse toolkit for investigating RNA dynamics and function within live cells, thereby contributing to advancements in our understanding of cellular processes at the molecular level.

Live-Cell Imaging of mRNA Using a Pepper RNA Tag.- A Method for Bioluminescence-Based RNA Monitoring Using Split-Luciferase Reconstitution Techniques.- A Catalytic Hairpin Assembly-Based Approach for RNA Imaging in Living Cells.- Live Single-Cell Transcriptional Dynamics in Plant Cells.- Live-Cell RNA Imaging with a DNA-Functionalized Metal-Organic Framework-Based Fluorescent Probe.- 3D Walking Nanomachine for Monitoring of miRNA in Living Cells.- Live-Cell Imaging of RNA G-Quadruplex with a Dual-Color Fluorescence Switch-On Probe.- Visualization of Enlsq