MALDI-TOF and Tandem MS for Clinical Microbiology [Hardcover]

This book highlights the triumph of MALDI-TOF mass spectrometry over the past decade and provides insight into new and expanding technologies through a comprehensive range of short chapters that enable the reader to gauge their current status and how they may progress over the next decade.  This book serves as a platform to consolidate current strengths of the technology and highlight new frontiers in tandem MS/MS that are likely to eventually supersede MALDI-TOF MS. Chapters discuss:
Challenges of Identifying Mycobacterium to the Species level
Identification of Bacteroides and Other Clinically Relevant Anaerobes
Identification of Species in Mixed Microbial Populations
Detection of Resistance Mechanisms
Proteomics as a biomarker discovery and validation platform
Determination of Antimicrobial Resistance using Tandem Mass Spectrometry

List of Contributors xxi

Preface xxix

Part I MALDI-TOF Mass Spectrometry 1

1 A Paradigm Shift from Research to Front?]Line Microbial Diagnostics in MALDI?]TOF and LC?]MS/MS: A Laboratory’s Vision and Relentless Resolve to Help Develop and Implement This New Technology amidst Formidable Obstacles 3
Haroun N. Shah and Saheer E. Gharbia

2 Criteria for Development of MALDI?]TOF Mass Spectral Database 39
Markus Kostrzewa and Thomas Maier

3 Applications of MALDI?]TOF Mass Spectrometry in Clinical Diagnostic Microbiology 55
Onya Opota, Guy Prod’hom and Gilbert Greub

4 The Challenges of Identifying Mycobacterium to the Species Level using MALDI?]TOF MS 93

5 Transformation of Anaerobic Microbiology since the Arrivall³,