Mammalian Synthetic Promoters [Hardcover]

This volume details methods that will aid in the selection of promoter sequences and vector components and methods for the assembly and testing of synthetic promoters with examples of their application. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Mammalian Synthetic Promoters: Methods and Protocols aims to aid researchers that are new to the field of synthetic promoters and inspire new developments.

David Gould

 

Part I: Monitoring Promoter Interactions and Activity

2          Demonstrating Interactions of Transcription Factors with DNA by Electrophoretic Mobility Shift Assay

Nasim Yousaf and David Gould

 

3.         Chromatin Immunoprecipitation and Quantitative Real-time PCR to Assess Binding of a Protein of Interest to Identified Predicted Binding Sites within a Promoter

Jordan Read

 

4.         Secreted Reporters for Monitoring Multiple Promoter Function

Ghazal Lashgari, Rami S. Kantar, and Bakhos A. Tannous

 

5.         Bioluminescence Monitoring Of Promotl£Ù