Membrane Lipids: Methods and Protocols [Hardcover]

This detailed book explores examples of current in vitro and in silico techniques that are at the forefront of lipid membrane research today. Beginning with methods and strategies associated with the creation and use of lipid membrane models in various research settings, the volume continues with electrical impedance spectroscopy strategies and methods to identify how ions and proteins interact with model lipid bilayers, guidance on lipid bilayer in silico molecular dynamics modeling, novel techniques to explore lipid bilayer characteristics using neutron scattering, IR spectroscopy, and atomic force microscopy (AFM), as well as unique fluorescence techniques. Written in the highly successful Methods in Molecular Biology series style, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Methods for Forming Giant Unilamellar Fatty Acid Vesicles.- Preparing Ion Channel Switch Membrane-Based Biosensors.- Langmuir-Schaefer Deposition to Create an Asymmetrical Lipopolysaccharide Sparsely-Tethered Lipid Bilayer.- Electrochemical Impedance Spectroscopy as a Convenient Tool to Characterize Tethered Bilayer Membranes.- Measuring Voltage-Current Characteristics of Tethered Bilayer Lipid Membranes to Determine the Electro-Insertion Properties of Analytes.- Measuring Activation Energies for Ion Transport Using Tethered Bilayer Lipid Membranes (tBLMs).- Determining the Pore Size of Multimeric Peptide Ion Channels Using Cation Conductance Measures of Tethered Bilayerl#¼