Metastasis Research Protocols [Hardcover]

Diverse molecular, cellular, and environmental events must all come together to allow the successful formation of secondary cancers, metastases.? The second edition of?Metastasis Research Protocols,?brings together updated versions of the seminal technique that were presented in the first edition and also includes new techniques that have recently been shown to be important in illuminating the processes underlying this important area of biology.?Volume 2?presents?techniques applicable at the level of?living cells and tissues, and presents methodologies applicable to cell behaviour?in vitro, in animal models and in mathematical constructs. The aim is the study of ?the interaction between cancer cells and their host/environment. The focus throughout is on the tools that have been shown to be helpful in unravelling the processes important in cancer metastasis.? Written in the highly successful?Methods in Molecular Biology?series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

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Authoritative and practical,?Metastasis Research Protocols, Second Edition?seeks to aid scientists in the further study of new methods in the area of metastasis research.

In Vitro Invasion Assay Using Matrigel^TM - a ?Reconstituted Basement Membrane Preparation.- Single Cell and Spheroid Collagen Type I Invasion Assay.- Rocking Adhesion Assay System to Study Adhesion and Transendothelial Migration of Cancer Cells.- Small Cell Lung Cancer (SCLC) Cell Adhesion on E- and P-Selectin under Physiological Flow Conditions.- Adhesion of Tumour Cells to Matrices and Endothelium.- Cell Aggregation Assays.- Chick Heart Invasion Ass