Methods of Soil Enzymology provides the first comprehensive set of vetted methods for studying enzymes in soils. Readers will especially benefit from the step-by-step explanation of the lab procedures, as well as background information for using these methods effectively and analyzing data. Main topics include activity assays, enzyme extraction, and synthetic enzyme complexes. Each method covered includes background informaton, step-by-step descriptions of the procedure, and special comments regarding nuances, pitfalls, and interpretation of the method. Learn the latest research methods, including enzyme extraction methods and procedures for creating synthetic enzyme complexes, as well as the newest ways to use small-scale and high-throughput methods for enzyme activity assays. Written for the researcher, but welcoming to those new to soil enzymology, the introduction includes conceptual information to orient those who are not familiar with these methods but want to use them. In the tradition of SSSA methods books, Methods of Soil Enzymology features a comprehensive approach with a focus on ease of use.Foreword ix
Preface xi
Contributors xv
Conversion Factors for SI and Non-SI Units xvii
Chapter 1 A Brief History of Soil Enzymology Research 1
Richard P. Dick and Richard G. Burns
Chapter 2 Ecology of Extracellular Enzyme Activities and Organic Matter Degradation in Soil: A Complex Community-Driven Process 35
Matthew D. Wallenstein and Richard G. Burns
Chapter 3 Kinetics of Soil Enzyme Reactions 57
Warren A. Dick
Chapter 4 Development of a Soil Enzyme Reaction Assay 71
Warren A. Dick
Chapter Sampling and Pretreatment of Soil before Enzyme Analysis 85
Nicola Lorenz and Richard P. Dick<l3%