Monoclonal Antibodies: The Second Generation [Hardcover]

A review of what needs to be done to realise the potential of monoclonal antibodies. The book assesses the competing technologies with advice on the best approach for a particular situation.Contributors -- Abbreviations -- Preface -- Safety note -- 1. Introduction. H. Zola -- Introduction: the purpose of this book -- Monoclonal antibodies: scope and limitations -- Antibodies as laboratory reagents: a brief perspective -- Antibodies as therapeutic agents: a brief perspective -- Conclusions -- References -- 2. Making and using 'conventional' mouse monoclonal antibodies. P.J. Donohoe, P.J. Macardle and H. Zola -- Introduction -- Tissue culture -- Materials -- Working solutions -- The screening assay -- Immunization -- The myeloma cell line -- Fusion protocol -- Feeder cells -- Post-fusion care -- Mycoplasma -- Cloning -- Cryopreservation -- Thawing cells out -- Ascites fluid -- Production of monoclonal antibodies in dialysis tubing -- Continuous perfusion culture systems -- Purification -- Conjugation -- References -- 3. Human monoclonal antibodies from immortalized B cells. A. W. Hohmann, L. Spatz, M. Irigoyen and A. Manheimer-Lory -- Introduction -- Immortalized B cells to probe the human antibody repertoire -- Infectious diseases -- Human immunodeficiency virus -- Erythrocyte antigens -- Human histocompatibility antigens -- Tumor antigens -- Autoantibodies -- Immortalization of B cells with EBV -- EBV transformation of B cells: methodology -- Making human hybridomas -- Hybridoma methodology -- References -- 4. Reconstruction of monoclonal antibodies by genetic engineering. J.R. Adair and D.J. King -- Introduction -- The reconstruction process -- The binding site -- The effector functions -- Reconstructed antibodies in practice -- Summary and conclusions -- References -- 5. The genetic engineering of antibody constructs for diagnosis and therapy. G.A. Pietersz and l.F.C. McKenzie -- Introduction -- Principles of use of antibody conjugates -- Recent advances in immunolÏ