This volume presents readers with the latest techniques to study nanoimaging and nanoprobing in application to a broad range of biological systems. The chapters in this book are divided into five parts, and cover topics such as imaging and probing of biomacromolecules including high-speed imaging and probing with AFM; probing chromatin structure with magnetic tweezers; and fluorescence correlation spectroscopy on genomic DNA in living cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and through, Nanoscale Imaging: Methods and Protocols is a valuable resource for anyone interested in learning more about this developing and expanding field.
High Resolution Atomic Force Microscopy Imaging of Nucleic Acids.- Two Ligand Binding Sites in Serotonin Transporter Revealed by Nanopharmacological Force Sensing.- AFM-Based Single-Molecule Force-Spectroscopy of Proteins.- High Resolution AFM-Based Force Spectroscopy.- Polymer Nanoarray Approach for the Characterization of Biomolecular Interactions.- Measuring Single-Molecule Twist and Torque in Multiplexed Magnetic Tweezers.- AFM-Based Characterization of Electrical Properties of Materials.- Supported Lipid Bilayers for Atomic Force Microscopy Studies.- Quantifying Small Molecule Binding Interactions with DNA Nanostructures.- Optimum Substrates for Imaging Biological Molecules with High-Speed Atomic Force Microscopy.- High Resolution and High-Speed Atomic Force Microscope Imaging.- High-Speed Atomic Force Microscopy of Individual Amyloidogenic Protein AssemlC"
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