Neurostereology: Unbiased Stereology of Neural Systems [Hardcover]

Stereological methods provide researchers with unparalleled quantitative data from tissue samples and allow for well-evidenced research advances in a broad range of scientific fields. Presenting a concise introduction to the methodology and application of stereological research in neuroscience,?Neurostereology?provides a fuller understanding of the use of these methods in research and a means for replicating successful scientific approaches. Providing sound footing for future research,?Neurostereology?is a useful tool for basic and clinical researchers and advanced students looking to integrate these methods into their research.

Contributors ix

Preface xiii

1 Stereological Estimation of Brain Volume and Surface Area from MR Images 3
Niyazi Acer and Mehmet Turgut

Background 3

The Cavalieri Principle 3

Volume Estimation Using Spatial Grid of Points 7

Surface Area Estimation 8

Isotropic Cavalieri Design 14

Sections Analyzed with Independent Grid Design 14

Surface Area Using the Invariator 16

Volume and Surface Area Estimation Using Segmentation Method 17

Discussion 18

References 19

Webliography 21

Appendix A: R Commands for Point Counting Method 21

Appendix B: R Commands for Vertical Section to Estimate Surface Area 23

2 Cell Proliferation in the Brains of Adult Rats Exposed to Traumatic Brain Injury 27
Sandra A. Acosta, Naoki Tajiri, Paula C. Bickford, and Cesar V. Borlongan

Background 27

Materials and Methods 28

Results 29

DiscussiolC+