The Nuclear Envelope Methods and Protocols [Hardcover]

This volume provides a wide rangeof protocols used in studying the nuclear envelope, with special attention tothe experimental adjustments that may be required to successfully investigatethis complex organelle in cells from various organisms. The Nuclear Envelope:Methods and Protocols is divided into five sections: Part I  Nuclear EnvelopeIsolation; Part II  Nuclear Envelope Protein Interactions, Localization, andDynamics; Part III  Nuclear Envelope Interactions with the Cytoskeleton; PartIV  Nuclear Envelope-Chromatin Interactions; and Part V  Nucleo-CytoplasmicTransport. Many of the modifications discussed in this book have only beencirculated within laboratories that have conducted research in this field formany years. Written in the highly successful Methods in Molecular Biologyseries format, chapters include introductions to their respective topics, listsof the necessary materials and reagents, step-by-step, readily reproduciblelaboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting edge and thorough, The Nuclear Envelope: Methods and Protocols is a timely resource for researchers who have joined this dynamic and rapidly growing field. 

Part I Nuclear Envelope Isolation

1. Isolation,Proteomic Analysis and Microscopy Confirmation of the Liver Nuclear EnvelopeProteome
   Nadia Korfali, LaurenceFlorens, and Eric C. Schirmer
   
   
2. Exploringthe Protein Composition of the Plant Nuclear Envelope
   Xiao Zhou,Kentaro Tamura, Katja Graumann, and Iris Meier
   
   
3. High EfficiencyIsolation of Nuclear Envelope Protein Complexes from Trypanosomes
   Samson Obado, Mark C.Field, Brian T. Chal"