Nucleic Acids [Paperback]

In recent years there has been a tremendous increase in our understanding of the functioning of the cell at the molecular level. This has been achieved in the main by the invention and development of new methodology, parti- larly in that area generally referred to as genetic en- neering . While this revolution has been taking place in the field of nucleic acids research, the protein chemist has at the same time developed fresh methodology to keep pace with the requirements of present day molecular bi- ogy. Today's molecular biologist can no longer be content with being an expert in one particular area alone. He/she needs to be equally competent in the laboratory at h- dling DNA, RNA, and proteins, moving from one area to another as required by the problem he/she is trying to solve. Although many of the new techniques in molecular biology are relatively easy to master, it is often difficult for a researcher to obtain all the relevant information nec- sary for setting up and successfully applying a new te- nique. Information is of course available in the research l- erature, but this often lacks the depth of description that the new user requires. This requirement for in-depth pr- tical details has become apparent by the considerable - mand for places on our Molecular Biology Workshops held at Hatfield each summer.The Burton Assay for DNA.- DABA Fluorescence Assay for Submicrogram Amounts of DNA.- Preparation of RNase-Free DNase by Alkylation.- The Isolation of Satellite DNA by Density Gradient Centrifugation.- The Isolation of High Molecular Weight Eukaryotic DNA.- Preparation of Lyophilized Cells to Preserve Enzyme Activities and High Molecular Weight Nucleic Acids.- Agarose Gel Electrophoresis of DNA.- Autoradiography of Gels Containing 32P.- Detection of Specific DNA SequencesThe Southern Transfer.- The Extraction and Isolation of DNA from Gels.- One-Dimensional Electrophoresis of Nucleic Acids in Agarose Using Denaturation with Formaldehyde and IdentificationlCÁ