This volume provides an overview on design PCR primers for successful DNA amplification. Chapters focus on primer design strategies for quantitative PCR, in silico PCR primer design, and primer design using software. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, PCR Primer Design, Second Edition seeks to aid molecular biology students, researchers, professors and PCR enthusiasts.
1. Fast Masking Of Repeated Primer Binding Sites In Eukaryotic Genomes
Reidar Andreson, Lauris Kaplinski, and Maido Remm
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2. Primer Design for PCR Reactions in Forensic Biology
Kelly M. Elkins
?3. Design of Primers and Probes For Quantitative Real-Time PCR Methods
Alicia Rodr?guez, Mar Rodr?guez, Juan J. C?rdoba, and Mar?a J. Andrade
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?4. Large-scale Nucleotide Sequence Alignment And Sequence Variability Assessment To Identify the Evolutionarily Highly Conserved Regions For Universal Screening PCR Assay Design: An Example Of Influenza A Virus
Nagy Alexander,? JiYinec, Tom?a, JiYincov?, Helena, and Havl?1kov?, Martina
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5. Low-concentration Initiator Primers Improve The
?Amplification Of Gene Targets With High Sequence Variability
Kenneth E. Pierce and Lawrence J. Wangh
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6. Multiplex PCR Primer Design for Simultaneous Detection of Multiple Pathogens
Wenchao Yan
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7. Degenerate Primer Design for Highly Variable Genomes
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