Photodynamic Therapy: Methods and Protocols [Hardcover]

This collection explores state-of-the-art methods and protocols for research on photodynamic therapy (PDT) and its use in a wide range of medical applications, from antiviral to anticancer. Beginning with an extensive section on in vitro and in vivo models, the volume continues with chapters on oxygen-independent photosensitizers, next-generation photosensitization strategies, contemporary insights into the immunomodulatory effects of PDT, antimicrobial effects of PDT, as well as a variety of general biochemical and molecular biological techniques. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of detailed implementation advice that ensures successful results in the lab. Application of Monolayer Cell Cultures for Investigating Basic Mechanisms of Photodynamic Therapy.- The Negative Impact of Cancer Cell Nitric Oxide on Photodynamic Therapy.- Microtumor Models as a Preclinical Investigational Platform for Photodynamic Therapy.- A Microfluidic Model to Evaluate Photodynamic Therapy Treatment Response in 3D Tumors.- Analysis of Treatment Effects on Structurally Complex Microtumor Cultures Using a Comprehensive Image Analysis Procedure.- High-Throughput Examination of Therapy-Induced Alterations in Redox Metabolism in Spheroid and Microtumor Models.- Spatiotemporal Tracking of Different Cell Populations in Cancer Organoid Models for Investigations on Photodynamic Therapy.- Generating Large Numbers of Pancreatic Microtumors on Alginate-Gelatin Hydrogels for Quantitative Imaging of Tumor Growth and Photodynamic TherapylóÉ