This volume presents a comprehensive overview of contemporary methods to analyze photorespiration in higher plants. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Photorespiration: Methods and Protocols aims to ensure successful results in the further study of this vital field.
1. Estimation of Photorespiratory Fluxes by Gas Exchange
Florian A. Busch, Ross M. Deans, and Meisha-Marika Holloway-Phillips
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2. Measurement of Transcripts Associated with Photorespiration and Related Redox Signaling
Amna Mhamdi, Pavel I. Kerchev, Patrick Willems, Graham Noctor, and Frank Van Breusegem
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3. Measurement of Enzyme Activities
Hermann Bauwe
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4. In vitro Alkylation Methods for Assessing the Protein Redox State
Flavien Zannini, J?r?my Couturier, Olivier Keech, and Nicolas Rouhier
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5. Dimethyl-labeling Based Quantification of the Lysine Acetylome and Proteome of Plants
?Ines Lassowskat, Markus Hartl, Fabian Hosp, Paul J. Boersema, Matthias Mann, and ?Iris Finkemeier
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6. In vitro Analysis of Metabolite Transport Proteins
Marc-Sven Roell, Franziska Kuhnert, Shirin Zamani-Nour, and Andreas P.M. W
eber
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7. Quantification of Photorespiratory Intermediates by Mass Spectrometry-based Approaches
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