Photorespiration: Methods and Protocols [Hardcover]

This volume provides methods on researching photorespiration to provide a better understanding of this vital pathway in plants. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Photorespiration: Methods and Protocols aims to ensure successful results in the further study of this vital field.

High throughput phosphoglycolate phosphatase activity assay using crude leaf extract and recombinant enzyme to determine kinetic parameters Km and Vmax using a microplate reader-  Measuring phosphoglycolate phosphatase activity using NMR detection of glycolate.- Determination of phosphoglycolate phosphatase activity via a coupled reaction using recombinant glycolate oxidase.- Spectrophotometric Assays for Measuring Photorespiratory Glutamate:Glyoxylate and Serine:Glyoxylate Aminotransferase Reactions.- Mitochondrial dihydrolipoamide dehydrogenase (mtLPD1)  expression, purification, activity and redox regulation.-Spectrophotometric Assays for Measuring Hydroxypyruvate Reductase Activity.- High throughput glycerate kinase activity assay using crude leaf extract and recombinant enzyme to determine kinetic parameters K_m and V_max using a microplate reader.- Purification of recombinant N-terminal histidine-tagged Arabidopsis thalianaphosphoglycolate phosphatase 1, glycolate oxidase 1 and 2, and hydroxypyruvate reductase 1.- Measuring and Quantifying Characteristics of the Post-Illumination Burst.- Low-cost system for maintaining low atmospheric CO