Plant Gravitropism: Methods and Protocols [Hardcover]

This updated and expanded edition explores key methodologies to study the fascinating phenomenon of how plants readjust their growth toward gravity. In addition to the protocols delivering broad applications for gaining insight into other plant physiological processes, this new volume also focuses on techniques involving plants in space or the use of microgravity analogs to study plant biological phenomenon. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Plant Gravitropism: From Mechanistic Insights into Plant Function on Earth to Plants Colonizing Other Worlds.- Evaluation of Gravitropism in Non-Seed Plants.- Spore Preparation and Protoplast Isolation to Study Gravity Perception and Response in Ceratopteris richardii.- A Multiplexed, Time-Resolved Assay of Root Gravitropic Bending on Agar Plates.- Methods to Quantify Cell Division and Hormone Gradients During Root Tropisms.- Using the Automated Botanical Contact Device (ABCD) to Deliver Reproducible, Intermittent Touch Stimulation to Plants.- High-Resolution Kinematic Analysis of Root Gravitropic Bending Using RootPlot.- Staging of Emerged Lateral Roots in Arabidopsis thaliana.- Methods for a Quantitative Comparison of Gravitropism and Posture Control Over a Wide Range of Herbaceous and Woody Species.- The Analysis of Gravitropic Setpoint Angle Control in Plants.- A Flal#É