This book provides detailed protocols for research in plant photomorphogenesis. The collection includes a broad range of topics including assays for shade avoidance responses, assays for light-dependent protein-protein interactions, photobody detection with immunofluorescence and the super-resolution imaging method, protein complex isolation from plants, detection of homodimer and monomer of photoreceptor UVR8 with immunoblotting analysis, assays for seedling greening, procedures for studying skotomorphogenesis, phenotypic study of photomorphogenesis at the seedling stage, expression of Cryptochrome in insect cells, and more. Written for the highly successful
Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Plant Photomorphogenesis: Methods and Protocols serves as an ideal guide for researchers and students who are new to the field, as well as a stepping stone for experienced researchers to further their skills in this fast-developing field.
Setting up an Arabidopsis LED Culture Module that Simulates Plant Neighbor Proximity.- Photobody Detection Using Immunofluorescence and Super-Resolution Imaging in Arabidopsis.- Analysis of Shade-Induced Hypocotyl Elongation in Arabidopsis.- Isolation of UVR8 Protein Complexes.- Phenotypic Study of Photo-Morphogenesis in Arabidopsis Seedlings.- Experimental Procedures for Studying Skotomorphogenesis in
Arabidopsis thaliana.- Global Identification for Targets of Circadian Transcription Factors in Arabidopsis and Rice Using Chromatin Immunoprecipitation Followed by Sequencing (ChIP-seq).- Co-Immunoprecipitation Assays to Detect In Vivo Association of Phytochromelz
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