Plant Transposable Elements: Methods and Protocols [Paperback]

Transposable elements have played a major role in shaping plant genome structure and gene expression.? Transposons not only drive sequence expansion, induce mutations and generate chromosome rearrangements, they also help to shape the epigenetic topology of the eukaryotic genome.? In Plant Transposable Elements: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study transposons. These methods include computational approaches to study the ancient transposon remnants that comprise the bulk of plant genomes, as well as laboratory techniques to identify recent and ongoing transposition events. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

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Authoritative and practical, Plant Transposable Elements: Methods and Protocols seeks to aid scientists in the further study of transposons by providing essential background information and specific experimental protocols.

Historical Overview of Transposable Element Research.- Distinguishing Variable Phenotypes from Variegation Caused by Transposon Activities.- Using Transposons for Genetic Mosaic Analysis of Plant Development.- Survey of Natural and Transgenic Gene Markers Used to Monitor Transposon Activity.- Molecular Biology of Maize Ac/Ds Elements  an Overview.- Gene Tagging with Engineered Ds Elements in Maize.- Plant Regeneration Methods for Rapid Generation of a Large Scale of Ds Transposant Population in Rice.- Isolation of Sequences Flanking Ac Insertion Sites by Ac Casting.- Regl>