Practical Enzymology [Paperback]

A practice-oriented guide to assaying more than 100 of the most important enzymes, complete with the theoretical background and specific protocols for immediate use in the biochemical laboratory. Now expanded with a new section on metal ion determination.Praxisleitfaden f?r die Analyse von mehr als 100 der wichtigsten Enzyme, inkl. theoretischer Hintergrund und spezifische Protokolle f?r den direkten Einsatz im biochemischen Labor. Neuer Abschnitt zur Metallionenbestimmung.

Preface to the Third Edition xv

List of Abbreviations and Symbols xvii

1 General Aspects of Enzyme Analysis 1

1.1 Introduction and Essentials for Enzyme Assays 1

Standard Books, Series 4

Databases 4

1.2 Theoretical Basis of Enzyme Assays 4

1.2.1 Order of Enzyme Reactions 4

1.2.2 Importance of the Reaction Order for Enzyme Reactions 6

1.2.3 The Reaction Velocity, Significance, and Practical Aspects 10

1.2.3.1 Determination of the Reaction Velocity, the Progress Curve 10

1.2.3.2 Enzyme Units 15

1.2.3.3 A Short Discussion About Errors in Enzyme Assays 17

1.2.3.4 Practical Rules for the Preparation of Dilution Series 21

1.2.3.5 Statistical Treatment of Enzyme Reactions 23

1.2.4 Treatment of the MichaelisMenten Equation 24

1.2.4.1 General Considerations 24

1.2.4.2 Linear Representations of the MichaelisMenten Equation 26

1.2.5 Enzyme Inhibition 28

1.2.6 Multisubstrate Reactions 33

1.3 Essential Conditions for Enzyme Assays 35

1.3.1 Dependence on Solvents and Ionic Strength 35

1.3.2 pH Dependency 36

1.3.2.1 Isoelectric Point 3