Prenatal Gliogenesis in the Neopallium of the Rat [Paperback]

1 Introduction.- 1.1 Identification of Glial Cells.- 1.2 Histogenesis of the Neocortex.- 2 Material and Methods.- 2.1 Animals.- 2.2 Fixation.- 2.3 Standard Preparation of Tissues.- 2.4 Selection of Cortical Region for Analysis.- 2.5 3H-Thymidine Autoradiography.- 2.6 3H-GABA Autoradiography.- 2.7 Golgi Impregnation.- 2.8 Three-Dimensional Reconstructions of Electron Micrographs.- 2.9 Immunocytochemistry.- 2.10 Structural Criteria for Glial and Neuronal Cells.- 2.11 Text Structure and Terminology.- 3 Results.- 3.1 Histological Differentiation of the Neocortex.- 3.1.1 Early Marginal Zone (E13E14).- 3.1.2 Late Marginal Zone (E15E16).- 3.1.3 Subcortical Zone.- 3.1.4 Cortical Plate.- 3.1.5 Lamina I.- 3.1.6 Mesenchymal Structures.- 3.2 First Cells of the Marginal Zone.- 3.2.1 Shape of Cells at E14.- 3.2.2 Marginal Contact Cells.- 3.2.2.1 Pial End-feet.- 3.2.2.2 Perikaryon and Small Processes.- 3.2.3 Intercellular Contacts.- 3.3 Cells of Lamina I.- 3.3.1 Quiescent Presumptive Astrocytes (E 17E 19).- 3.3.1.1 Perikaryon.- 3.3.1.2 Pial Process.- 3.3.2 Other Nonneuronal Cells (E17E19).- 3.3.2.1 Dense Nucleus Cells.- 3.3.2.2 Intermediate Cells.- 3.3.3 Cells Containing GFAP and C1-Antigen (E18E19).- 3.3.4 Distinction between Neuronal and Nonneuronal Cells.- 3.3.5 GABA-Accumulating Cells.- 3.3.6 Nonneuronal Cells at E 20 and E 21.- 3.4 Cells of the Deep Layers.- 3.4.1 Deep Marginal Zone (E15E16).- 3.4.1.1 Polymorphic Cells and Mitotic Cells.- 3.4.1.2 Horizontal Neuron-like Cells.- 3.4.1.3 Cellular Processes.- 3.4.1.4 Intercellular Relations.- 3.4.2 Subcortical Zone (E 17E 22).- 3.4.2.1 Dense Polymorphic Cells.- 3.4.2.2 Large Glial Precursors.- 3.4.2.3 Light Horizontal Cells.- 3.4.2.4 Phagocytes.- 3.4.2.5 Cell Proliferation.- 3.4.2.6 GABA Accumulation.- 3.4.2.7 Demonstration of GFAP and C1-Antigen (E 18, E 22).- 3.4.2.8 Golgi Impregnation.- 3.5 Nonneuronal Cells of the Cortical Plate.- 4 Discussion.- 4.1 Anlage of the Palliai Zones.- 4.2 Early Glial Cells with Pial Contact.lÓ*