Protein Affinity Tags: Methods and Protocols [Paperback]

Protein Affinity Tags: Methods and Protocols provides researchers with the necessary information, tools, and strategy required for proper inquiry into a given proteins function and structure. Todays researchers can easily alter the amino acid sequence of any given protein, a powerful technology allowing for the precise engineering of specific proteins. Protein affinity tagging employs the use of known protein binding interactions in order to fish out a protein-of-interest or, with more advanced tags, a protein complex. This technology paired with recent advancements in DNA sequencing technology promises a future that is awash with novel genomic information  information that will not only expand our knowledge of the processes that govern life, but also fuel innovation that will undoubtedly conquer many of todays most salient health and environmental challenges. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Protein Affinity Tags: Methods and Protocols will be an invaluable resource to those seeking to employ protein affinity tags to study their biological system of interest.

1D4 - A Versatile Epitope Tag for the Purification and Characterization of Expressed Membrane and Soluble Proteins.- Affinity Purification of Heme-Tagged Proteins.- Purification of a Recombinant Protein with Cellulose-Binding Module 3 as the Affinity Tag.- Purification of E. coli Proteins Using a Self-Cleaving Chitin-Binding Affinity Tag.- Simplified Protein Purification Using an Autoprocessing, Inducible Enzyme Tag.- SUMO as a Solubility Tag and in vivo Cleavage of SUMO Fusion Proteins with Ulp1.- Rescuing Aggregation-Prone ló