Shotgun Proteomics: Methods and Protocols [Hardcover]

Shotgun Proteomics: Methods and Protocols serves as a vital collection of protocols through which thousands of proteins can be simultaneously identified, quantified and characterized in a high throughput manner. Beginning with the history of proteomics centered on the vital role of mass spectrometry in its development, this detailed volume continues with chapters on sample pre-fractionation, in vivo and in vitro stable isotope labeling, label-free proteomics, informatics, protein-protein interactions, targeted proteomics and post-translational modifications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Practical and comprehensive, Shotgun Proteomics: Methods and Protocols is an ideal and up-to-date guide for researchers seeking to understand the proteome of any given species.

Survey of Shotgun Proteomics.- LC-MALDI-TOF/TOF for Shotgun Proteomics.- Fully Automatable Multidimensional Reversed-Phase Liquid Chromatography with Online Tandem Mass Spectrometry.- GeLC-MS/MS Analysis of Complex Protein Mixtures.- IPG Strip-Based Peptide Fractionation for Shotgun Proteomics.- SILAC Yeast: From Labeling to Comprehensive Proteome Quantification.- Analysis of Proteome Dynamics in Mice by Isotopic Labeling.- Stable Isotope Labeling in Mammals (SILAM).- Analysis of Individual Protein Turnover in Live Animals on a Proteome-Wide Scale.- Determining Protein Subcellular Localization in Mammalian Cell Culture with Biochemical Fractionation and iTRAQ 8-Plex Quantification.- Brain Quantitative Proteomics Combining GeLC-MS and Isotope-Coded Protein Labeling (ICPL).- Employing TMT Quantification in a Shotgun-MS Platform.- Employing TMT QuantificatilóÉ