Stem Cells and Good Manufacturing Practices: Methods, Protocols, and Regulations [Paperback]

This detailed book explores the utilization and delivery of stem cells for therapeutic purposes in patients in the clinic and the tightly controlled Good Manufacturing Practices (GMP) that make these powerful techniques possible. Fully updated and expanded from the first edition, this collection features a new set of protocols to arm stem cell biologists with protocols that are currently being used in a number of well-established facilities around the world. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Swiss Fetal Transplantation Program and Non-Enzymatically Isolated Primary Progenitor Cell Types for Regenerative Medicine.- GMP Tiered Cell Banking of Non-Enzymatically Isolated Dermal Progenitor Fibroblasts for Allogenic Regenerative Medicine.- Progenitor Biological Bandages: An Authentic Swiss Tool for Safe Therapeutic Management of Burns, Ulcers, and Donor Site Grafts.- Validation of the Media Fill Method for Solid Tissue Processing in Good Manufacturing Practice-Compliant Cell Production.- The Isolation, Culturing, Cryopreservation, and the Preparation as a Final Cellular Product of the Umbilical Cord-Derived Mesenchymal Stem Cells Under Good Manufacturing Practices Conditions.- The Isolation, Culture, Cryopreservation, and the Preparation as a Final Cellular Product of the Skin-Derived Fibroblasts Under Good Manufacturing Practice Conditions.- The Isol#)