Th9 Cells Methods and Protocols [Hardcover]

This volume presents comprehensive laboratory protocols that have been used to generate Th9 cells, both in vitro and in vivo. The techniques described in Th9 Cells: Methods and Protocols study the role of Th9 cells in different inflammatory disease models, including allergic inflammation model, parasite model, tumor model, and EAE and IBD model. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and comprehensive, Th9 Cells: Methods and Protocols is a valuable resource for scientists in this field and will provide them with techniques to generate Th9 cells for specific downstream events.

Preface&
Table of Contents&
Contributing Authors..

1. Th9 Cells: New Member of T Helper Cell Family
Ritobrata Goswami

2. IL-9: Function, Sources, and Detection
Wilmer Gerardo Rojas-Zuleta and Elizabeth Sanchez

3. IL-9 Signaling Pathway: An Update
Dijendra Nath Roy and Ritobrata Goswami

4. A Method to In Vitro Differentiate Th9 Cells from Mouse Na?ve CD4+ T Cells
Duy Pham

5. T Cell Receptor and Co-Stimulatory Signals for Th9 Generation
Fran?oise Meylan and Julio Gomez-Rodriguez

6. Polarizing Cytokines for Human Th9 Cell Differentiation
Prabhakar Putheti

7. Determining the Frequencies of Th9 Cells from Whole Blood
Anuradha Rajamanickam and Subash Babu

8. IL-9 Production by Nonconventional T Helper Cells
Silvia C. P. Almeida and Luis Graca
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