Vaccine Design Methods and Protocols Volume 1 Vaccines for Human Diseases [Hardcover]

This text provides a practical guide providing step-by-step protocol to design and develop vaccines. Chapters detail protocols for developing novel vaccines against infectious bacteria, viruses, fungi, and parasites for humans and animals. Volume 1: Vaccines for Human Diseases has an introductory section on how vaccines impacted diseases, the immunological mechanism of vaccines, future challenges for vaccinologists, and current trends in vaccinology. The design of human vaccines for viral, bacterial, fungal, parasitic and prion diseases as well as vaccines for drug abuse, allergy, and tumor vaccines are also described in this volume. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.

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Authoritative and practical, Vaccine Design: Methods and Protocols, Volume 1: Vaccines

for Human Diseases aims to ensure successful results in the further study of this vital field.

Clinical Impact of Vaccine Development.- Future Challenges for Vaccinologists.- Principles of Vaccination.- Reverse Vaccinology: The Pathway from Genomes and Epitope Predictions to Tailored Recombinant Vaccines.- Systems Vaccinology: Applications, Trends, and Perspectives.- Proteomic Monitoring of B Cell Immunity.- Development of Rabies Virus-Like Particles for Vaccine Applications: Production, Characterization and Protection Studies.- Analytic Vaccinology: Antibody-Driven Design of a Human Cytomegalovirus Subunit Vaccine.- Generation of a Single-Cycle Replicable Rift Valley Fever Vaccine.- Application of Droplet Digital PCR to Validate Rift Valley Fever Vaccines.- Methods to Evaluate Novel Hepatitis C Virus VlCÏ