Vaccine Design: Methods and Protocols, Volume 1. Vaccines for Human Diseases [Paperback]

This volume provides a practical guide providing step-by-step protocol to design and develop vaccines for human diseases. Divided into three volumes, Volume 1: Vaccines for Human Diseases guides readers through an introductory section on future challenges for vaccinologists and the immunological mechanism of vaccines. Chapters focus on design of human vaccines for viral, bacterial, fungal, and parasitic diseases as well as tumor vaccines. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

Authoritative and practical, Vaccine Design: Methods and Protocols, Second Edition, Volume 1: Vaccines for Human Diseases aims to be a useful practical guide to researchers to help further their study in this field.

Challenges for Vaccinologists in the First Half of the Twenty First Century.- Principles in Immunology for the Design and Development of Vaccines.- Revisiting the Principles of Designing a Vaccine.- Status of COVID-19 Pandemic before the Administration of Vaccine.- mRNA Vaccines to Protect against Diseases.- Artificial Intelligence in Vaccine and Drug Design.- Vaccines Targeting Numerous Coronavirus Antigens, Ensuring Broader Global Population Coverage: Multi-Epitope and Multi-Patch Vaccines.-Use of Micro-computed Tomography to Visualize and Quantify COVID-19 Vaccine Efficiency in Free-breathing Hamsters.- Design of Replication-Competent VSV- and Ervebo-vectored Vaccines against SARS-CoV-2.- CRISPR Engineering of Bacteriophage T4 to Design Vaccines against SARS-CoV-2 and Emerging Pathogens.- Techniques for Developing and Assessingl1