Zebrafish: Methods for Assessing Drug Safety and Toxicity [Hardcover]

Consolidating key protocols and approaches to help researchers navigate the important and evolving field of zebrafish models for toxicity screening, this new title describes the methods for using the zebrafish as a model organism to assess compound-induced toxicity on all major organs.? Individual chapters that concentrate on assays for each organ system are included and various analytical tools including microscopy, microplate readers, high content imaging systems, ECG, blood pressure monitors, high speed video and motion detectors are described.

Contributors xiii

Acknowledgments xvii

1. The Reproductive Biology and Spawning of Zebrafish in Laboratory Settings 1

1.1 Introduction 1

1.2 Overview of Zebrafish Reproductive Biology and Behavior 2

1.3 Spawning Techniques and Technology 5

1.4 Determining Factors for Reproduction in Laboratory Stocks of Zebrafish 8

1.5 Conclusions 11

References 11

2. Developmental Toxicity Assessment in Zebrafish 15

2.1 Introduction 15

2.2 Methods 16

2.3 Results 20

2.4 Discussion 23

References 25

3. Use of Emerging Models for Developmental Toxicity Testing 27

3.1 Importance of Assessing Developmental Toxicity 27

3.2 Current Methods for Assessing Developmental Toxicity 27

3.3 Use of Emerging Models for Developmental Toxicity Testing 28

3.4 New Guidelines for Chemical Testing Using Zebrafish 29

3.5 Conclusions 42