Zebrafish: Methods and Protocols [Hardcover]

This fully updated edition introduces new tools, models, and analytic insights that position the zebrafish even more strongly as an engine of discovery for developmental and disease biology. Beginning with a section exploring detailed methods for use of zebrafish to model a variety of human diseases, the book continues by illuminating the key ongoing role of the fish model in studies of the vertebrate nervous system, tools and approaches using zebrafish to study stem cell and regenerative biology, as well as techniques in genetics and genomics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls. Delivering Traumatic Brain Injury to Larval Zebrafish.- Functional Genomics of Novel Rhabdomyosarcoma Fusion-Oncogenes Using Zebrafish.- Methods to Study Liver Disease Using Zebrafish Larvae.- Developmental Toxicity Assessment Using Zebrafish-Based High Throughput Screening.- Cancer Modeling by Transgene Electroporation in Adult Zebrafish (TEAZ).- Lineage Tracing of Bone Cells in the Regenerating Fin and during Repair of Bone Lesions.- Primary Culture of Neuronal Populations for Various Downstream Applications.- Holographic Optogenetic Activation of Neurons Eliciting Locomotion in Head-Embedded Larval Zebrafish.- Brain Imaging and Registration in Larval Zebrafish.- Simultaneous Behavioral and Neuronal Imaging by Tracking Microscopy.- Genetic Identification of Neural Circuits Essential for Actil#