3D Cell Culture Methods and Protocols [Hardcover]

This book provides an overview of established 3D cell culture assays from leaders in the field. Their contributions cover a wide spectrum of techniques and approaches for 3D cell culture, from organoid cultures through organotypic models to microfluidic approaches and emerging 3D bioprinting techniques, which are used in developmental, stem cell, cancer, and pharmacological studies, among many others. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 1. 3D Cell Culture: An Introduction

            Zuzana Koledova

 

Part I: Hydrogels and Scaffolds for 3D Cell Culture

 

<2. Preparation of Decellularized Biological Scaffolds for 3D Cell Culture

            Bryan N. Brown, Michael J. Buckenmeyer, and Travis A. Prest