Adipose-Derived Stem Cells Methods and Protocols [Hardcover]

During the past decade, a wide range of scientific disciplines have adopted the use of adipose-derived stem/stromal cells (ASCs) as an important tool for research and discovery. In Adipose-Derived Stem Cells: Methods and Protocols, experts from the field, including members of the esteemed International Federation of Adipose Therapeutics and Science (IFATS), provide defined and established protocols in order to further codify the utilization of these powerful and accessible cells. With chapters organized around approaches spanning the discovery, pre-clinical, and clinical processes, much of the emphasis is placed on human ASC, while additional techniques involving small and large animal species are included. As a volume in the highly successful Methods in Molecular Biology series, the detailed contributions include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.Comprehensive and cutting-edge, Adipose-Derived Stem Cells: Methods and Protocols serves as a vital reference text for experienced researchers as well as new students on the path to further exploring the incredible potential of ASCs.

This comprehensive reference features experts from the field, including members of the International Federation of Adipose Therapeutics and Science (IFATS), who provide well defined protocols that further codify the applications of these powerful cells.

Part I: Isolation Methods1. Isolation and Culture of Rhesus Adipose-Derived Stem CellsChristine Gagliardi and Bruce A. Bunnell2. Isolation of Human Adipose-Derived Stem Cells from LipoaspiratesGang Yu, Z. Elizabeth Floyd, Xiying Wu, Yuan-Di C. Halvorsen, and Jeffrey M. Gimble3. Isolation of Murine Adipose-Derived Stem CellsGang Yu, Xiying Wu, Gail Kilroy, Yuan-Di C. Halvorsen, Jeffrey M Gimble, and Z. Elizabeth Floyd4. In vitro Adult Rat Adipose Tissue-Derivedlc¶