ADP-ribosylation and NAD+ Utilizing Enzymes Methods and Protocols [Hardcover]

This volume focuses on mono-ADP-ribosylation and enzymes that use NAD+ including Sirtuins, PARPs, and bacterial and eukaryotic ADP-ribosyltransferases. The chapters in this book are organized into eight parts, and offer detailed descriptions of key protocols used to study topics such as in vitro techniques for ADP-ribosylation substrate identification; biochemical and biophysical assays of PAR-WWE domain interactions; monitoring expression and enzyme activity of ecto-ARTCs; HPLC-based enzymes assays for Sirtuins; and identifying target RNAs of PARPs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, ADP-ribosylation and NAD+ Utilizing Enzymes: Methods and Protocols is a valuable resource for anyone interested in this developing and expanding field.

Preface&

Table of Contents&

Contributing Authors&

Part I Introduction

1. Vitamin B3 in Health and Disease: Toward the Second Century of Discovery

Myron K. Jacobson and Elaine L. Jacobson

Part II ADPribose Binding Domains as Tools

2.Monitoring Poly(ADP-Ribosyl)ation in Response to DNA Damage in Live Cells Using Fluorescently-Tagged Macrodomains

Rebecca Smith and Gyula Timinszky

3.In Vitro Techniques for ADP-Ribosylated Substrate Identification

Giovanna Grimaldi, Giulianan Catara, Carmen Valente, and Daniela Corda

4.Assessment of Intl'