Advanced Fluorescence Microscopy Methods and Protocols [Hardcover]

This volume provides an overview of advanced ?fluorescence microscopy, covering a broad range of methods. Each chapter focuses on a different method and provides a practical guide for application in biological systems.? Written in the highly successful Methods in Molecular Biology ?series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge,? Advanced Fluorescence Microscopy: Methods and Protocols seeks to provide scientists with methods for biological systems that are of interest.

1. The Physical Basis Of Total Internal Reflection Fluorescence (TIRF) Microscopy and Its Cellular Applications

Natalie S. Poulter, William T.E. Pitkeathly, Philip J. Smith, and Joshua Z. Rappoport

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2. Two-Photon Excitation Microscopy And Its Applications In Neuroscience

Ricardo Mostany, Amaya Miquelajauregui, Matthew Shtrahman, and Carlos Portera-Cailliau

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Francesco Pampaloni, Roli Richa, Nariman Ansari, and Ernst H.K. Stelzer

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4. Fluorescence Microscopy Based RNA Interference Screening

Manuel Gunkel, Nina Beil, J?rgen Beneke, J?rgen Reymann, and Holger Erfle

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5. Fluorescence Resonance Energy Transfer Microscopy (FRET)

Kasia M. Kedziora and Kees Jalink

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6. Localizing Protein-Protein Interactions In Living Cells Using Fluorescence Lifetime Imaging Microscopy

Yuansheng Sun, Vinod Jyothikumar, and Ammasi Periasamy

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