The aim of this volume is to describe the latest advances in microscopic methods, including integrated techniques, as applied to mycology. Each chapter will provide a brief overview of a particular microscopic method with associated advantages and limitations, the research questions that can be appropriately addressed using these microscopic methods, how it has been successfully applied to address mycological research questions, including supporting and complimentary techniques, and which future questions can be addressed.
Chapter 1. Applications of Confocal Laser Scanning Microscopy in Filamentous Fungi
Rosa R. Mouri?o-P?rez, MD, PhD and Robert W. Roberson, MD
Chapter 2. Fluorescence-based Methods for the Study of Protein Localization, Interaction, and Dynamics in Filamentous Fungi
Oier Etxebeste, PhD and Norio Takeshita, PhD
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Chapter 3. Super Resolution Microscopy: SIM, STED and Localization Microscopy
James Dodgson, PhD, Anatole Chessel, PhD, Susan Cox, PhD, and Rafael Carazo Salas, PhD
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Chapter 4. Fourier Transform Infrared (FTIR) Microscopy and Imaging of Fungi
Annette Naumann, Dipl. Biol.
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Chapter 5. Whole Cells Imaged by Hard X-ray Transmission Microscopy
Zhiting Liang, BA, Yong Guan, MD, Shan Chen, BA, and Yangchao Tian, MD
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Chapter 6. In situ Nanocharacterization of Yeast Cells Using ESEM and FIB
Yajing Shen, PhD
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Chapter 7. Imaging Living Yeasts Cells and Quantifying Their Biophysical Properties by Atomic Force Microscopy
C?cile Formosa and Etienne Dague