Alcohol Methods and Protocols [Paperback]

This book examines the pleiotropic effects of ethanol in animal and cell culture models through a collection of detailed procedures written by experts in the field. Sections present clearly defined models of ethanol exposure, recent advances in the development of specific methodologies to mimic the impact of ethanol metabolism in cultured cells, and methodologies to investigate a variety of cells and tissues that are known to be disrupted by ethanol, amongst other topics.

Many unexplored complex cellular and organismal adaptations occur in response to the stress of alcohol exposure, and its contribution to the development of chronic diseases, such as osteoporosis, heart disease and diabetes, is particularly relevant today, given the increased incidence of these diseases in our aging population. In Alcohol: Methods and Protocols, the pleiotropic effects of ethanol in animal and cell culture models are rigorously examined through a collection of detailed procedures written by experts in the field. Sections present clearly defined models of ethanol exposure, recent advances in the development of specific methodologies to mimic the impact of ethanol metabolism in cultured cells, and methodologies to investigate a variety of cells and tissues that are known to be disrupted by ethanol, amongst other topics.

Contents Preface Contributors Part I. Animal Models of Acute and Chronic Ethanol Exposure Acute models of ethanol exposure to mice Timothy P. Plackett and Elizabeth J. Kovacs A voluntary oral-feeding rat model for pathological alcohol liver injury George L. Tipoe, Emily C. Liong, Tung-Ming Leung,and Amin A. Nanji Intragastric ethanol infusion model in rodents Hide Tsukamoto, Hasmik Mikrtchyan and Alla Dynnyk A practical method of chronic ethanol administration in mice Ruth A Coleman, Betty M. Young, Lucas E. Turner and Robert T. Cook Part II. Animal models of ethanol exposure during development Analysis of ethanol devel#.