Animal Cell Culture Techniques [Paperback]

Cell culture techniques allow a variety of molecular and cell biological questions to be addressed, offering physiological conditions whilst avoiding the use of laboratory animals. In addition to basic techniques, a wide range of specialised practical protocols covering the following areas are included: cell proliferation and death, in-vitro models for cell differentiation, in-vitro models for toxicology and pharmacology, industrial application of animal cell culture, genetic manipulation and analysis of human and animal cells in culture.Cell culture techniques allow a variety of molecular and cell biological questions to be addressed, offering physiological conditions whilst avoiding the use of laboratory animals. In addition to basic techniques, a wide range of specialised practical protocols covering the following areas are included: cell proliferation and death, in-vitro models for cell differentiation, in-vitro models for toxicology and pharmacology, industrial application of animal cell culture, genetic manipulation and analysis of human and animal cells in culture.Animal Cell Culture Techniques (Springer Lab Manual), Clynes Ed. 1 Cloning Animal Cells, Shirley McBride, Mary Heenan, Martin Clynes. 2 Cell Culture and Diagnostic Virology, Edwin OKelly. 3 Screening for Mycoplasma Contamination in Animal Cell Cultures, Mary Heenan and Martin Clynes. 4 Serum-Free Media, Joanne Keenan, Paula Meleady, and Martin Clynes. 5 Screening Culture Media and Sera for Endotoxin Testing, Joanne Keenan and Martin Clynes. 6 Generation of Monoclonal Antibodies and Characterization of Novel Antibodies by Western Blotting and Immunocytochemistry, Elizabeth Moran, Annemarie Larkin, Allan Masterson, and Martin Clynes. 7 The Generation of Long-Term Human Antibody-Producing Cell Lines Using the Epstein-Barr Virus, Olivia Flynn and Dermot Walls. 8 Primary Culture, Finbar O Sullivan, Paula Meleady, Shirley McBride, and Martin Clynes. 9 Baculoviral Expression and Partial Purification of CyclCD