This detailed volume provides diagnosticians and researchers with practical methodologies and approaches to tackle animal coronaviruses. It explores conventional immunohistochemistry, virus neutralization, enzyme-linked immunosorbent assays, expression and purification of recombinant viral proteins, and various molecular assays, including conventional and real-time reverse transcription-PCR, reverse genetics methodology, and next generation sequencing and sequence analyses. As part of the Springer Protocols Handbooks series, chapters contain readily reproducible laboratory protocols as well as expert tips on troubleshooting and avoiding known pitfalls.?
Practical and authoritative,
Animal Coronaviruses serves as an ideal reference for researchers examining a wide variety of coronavirus species in the
Coronaviridae.
Part I: Introduction
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1. Animal Coronaviruses: A Brief Introduction
??????????? Leyi Wang and Yan Zhang
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Part II: Conventional Technologies
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2. Immunohistochemical Staining for Detection of Porcine Epidemic Diarrhea Virus in Tissues
??????????? Kwonil Jung
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3. Virus Neutralization Assay for Turkey Coronavirus Infection
??????????? Yi-Ning Chen, Ching Ching Wu, and Tsang Long Lin
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4. Recombinant Turkey Coronavirus Nucleocapsid Protein Expressed in Escherichia coli
??????????? Chien Chang Loa, Ching Ching Wu, and Tsang Long Lin
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5. Antibodyl#•
![Animal Coronaviruses [Hardcover]](/itemImage/2/1/4/7/2_753754.jpg "Animal Coronaviruses [Hardcover] xxx")