Animal Endo-SiRNAs Methods and Protocols [Hardcover]

Animal Endo-SiRNAs: Methods and Protocols presents a variety of approaches to investigate endo-siRNAs.?These include protocols applicable to study short RNAs expressed at a low level and model systems that are particularly suitable to investigate specific aspects of endo-siRNAs, their synthesis, their genomics or regulatory role. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Animal Endo-SiRNAs: Methods and Protocols contains practical tips that are absent in standard lab manuals.

1. Targeted Small Non-coding RNA Directed Gene Activation in Human Cells

Caio Damski and Kevin V. Morris

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2. Isolation of Chromatoid Dodies from Mouse Testis as a Rich Source of Short RNAs

Oliver Meikar and Noora Kotaja

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3. Generation of Endo-siRNAs in Xenopus laevis Oocytes

Sammer Alnumeir and Andreas Werner

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4. Analysis of Endo-siRNAs in Drosophila

Katharina Elmer, Stephanie Helfer , Milijana Mirkovic-H?sle and Klaus F?rstemann

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5. Methods for Studying the Biological Consequences of Endo-siRNA Deficiency in Drosophila melanogaster

Do-Hwan Lim, Chun-Taek Oh, Sung-Jun Han, and Young Sik Lee

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6. Small RNA Library Cloning Procedure for Deep Sequencing of Specific Endogenous siRNA Classes in Caenorhabditis elegans

Maria C. Ow, Nelson C. Lau, and Sarah Elƒ‚